The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified ATF2 is greater than 90% homogeneous based on SDS-PAGE analysis.
Unit Definition (Activity)
1 unit equals 1 nanogram of purified protein.
Applications
Recombinant ATF2 (1-505) can be used for in vitro phosphorylation, dephosphorylation assays and inhibitor screening assay. For Research Purposes Only.
Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Synonym
Homo sapiens activating transcription factor 2, CRE-BP1; CREB2; HB16; MGC111558 and TREB7.
The activating transcription factor 2 (ATF2), also known as CRE-BP1 (cAMP-responsive element binding protein 1), is a member of the leucine zipper family of DNA binding proteins and binds to both AP-1 and CRE DNA response elements of many viral and cellular promoters (1). ATF2 can be activated by a diverse number of cellular stresses such as inflammatory cytokines, genotoxic agents and UV irradiation through MAPK pathway (2-4). It is activated through phosphorylation at two phosphosites of Thr69 and Thr71 by upstream activators like JNK/p38 (MAPK14, SAPK2A) (2-4).
References
1. Abdel-Hafiz, HA etal (1992) Mol. Endocrinol. 6, 2079-2089. 2. Sano Y et al (1999) J Biol Chem 274: 8949-8957 3. Livingston C et al (1995) EMBO J 14:1785-1797
4. Kim SJ et al (1992) Nature 358:331-334
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
