MY ACCOUNT


BRG1 PACKAGE

$405.00
Size
Packaging Requirements for Dry Ice
In stock
Product Details

BRG1 Package Includes

  • wild type BRG1 (p1041-01) 5,000 units
  • ATPase deficiency mutation of BRG1 (p1042-01) 5,000 units


BRG1 is an essential component of the SWI/SNF chromatin remodeling complexes and is implicated in multiple functions through its interaction with different proteins, including the tumor suppressor protein pRb (Cat#P2007) (1), serine-threonine kinase LKB1 (2), and other transcription factors (3,4). Mutation of lysine to arginine at position 798 in the DNA-dependent ATPase domain of BRG1 a) failed to restore normal growth to swi2- cells (5), b) reduced its ability to repress c-fos transcription (6), and c) reduced CIITA (class II transactivator) induction and enhanced the rate of induction of the interferon-g-responsive GBP-1 gene (7).

Recombinant BRG1 (K798R) can be used 1) for protein-protein interaction assay; 2) for in vitro transcription assay; 3) for in vitro nucleosome remodeling assay; and 4) for cell growth assay.

Purified protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram purified protein. 100 units are required for a protein-protein interaction assay.
Concentration is variable in different lots

Supplied in 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA

References:
1. Dunaief, J.L. et al., (1994) Cell 79, 119-130
2. Marignani, P.A. et al., (2001) J. Biol. Chem. 276, 32415-32418
3. Brockmann, D. et al., (2001) Gene 277, 111-120
4. Barker, N. et al., (2001) EMBO J. 20, 4935-4943
5. Khavari, P.A. et al., (1993) Nature 366, 170-174
6. Murphy, D.J. et al., (1999) Mol. Cell. Biol. 19, 2724-2733
7. Pattenden, S.G. et al., (2002) EMBO J. 21, 1978-1986




DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.

Save this product for later
Share this product with your friends
BRG1 PACKAGE