CTF1(CCAAT-box-binding Transcription Factor 1) (Cat# P1035)

• Species; Human
• Expression Host: Baculovirus-insect cell
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 56.4 kDa.
• Gene Accession Number: X12492.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified CTF1 is greater than 90% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 30-100 units (ng) are required for a reconstituted transcription assay and 5-20 units (ng) are required for a gel mobility shift assay in a 20µl reaction.

Applications

Purified CTF1 has been used for in vitro transcriptional activation, DNase I protection, gel mobility shift and protein-protein interaction assays. For Research Use Only.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

CTF/NFI or CTF or NF-I or NF-1

Protein Sequence

MDEFHPFIEA LLPHVRAFAY TWFNLQARKR KYFKKHEKRM SKDEERAVKD ELLGEKPEVK QKWASRLLAK LRKDIRPECR EDFVLSITGK KAPGCVLSNP DQKGKMRRID CLRQADKVWR LDLVMVILFK GIPLESTDGE RLVKAAQCGH PVLCVQPHHI GVAVKELDLY LAYFVRERDA EQSGSPRTGM GSDQEDSKPI TLDTTDFQES FVTSGVFSVT ELIQVSRTPV VTGTGPNFSL GELQGHLAYD LNPASTGLRR TLPSTSSSGS KRHKSGSMEE DVDTSPGGDY YTSPSSPTSS SRNWTEDMEG GISSPVKKTE MDKSPFNSPS PQDSPRLSSF TQHHRPVIAV HSGIARSPHP SSALHFPTTS ILPQTASTYF PHTAIRYPPH LNPQDPLKDL VSLACDPASQ QPGPLNGSGQ LKMPSHCLSA QMLAPPPPGL PRLALPPATK PATTSEGGAT SPTSPSYSPP DTSPANRSFV GLGPRDPAGI YQAQSWYLG

Background

The CCAAT-box binding protein 1 (CTF1, also called NF-I or NF-1) is a proline-rich transcription activator (1,2). It selectively activates transcription of genes that contain GCCAAT consensus sequence in promoter region, including human Ha-Ras, alpha-globin, hsp70 and many other cellular and viral genes (1, 3-5). Cloning and functional characterization of the protein revealed that CTF1 contains an N-terminal DNA binding domain and a C-terminal proline-rich activation domain (2,3). CTF1 was shown to interact with the general transcription factor IIB (TFIIB) and facilitate the recruitment of TFIIB to TBP-DNA complexes (6), with the forkhead thyroid transcription factor TTF2 to regulate hormone–dependent transcription of thyroperoxidase gene (7), as well as other receptor proteins to synergize hormone receptor-responsive activation (8-10).

References

1. Johns, KA. et al., (1987) Cell 48, 79-89
2. Santoro, C. et al., (1988) Nature 334, 218-224
3. Mermod, N. et al., (1989) Cell 58, 741-753
4. Lum, LS et al., (1990) Mol. Cell. Biol. 10, 6709-6717
5. Cardinaux, JR. et al., (1994) J Biol. Chem. 269, 32947-32956
6. Kim, TK. et al., (1994) Proc. Natl. Acad. Sci. USA 91, 4170-4174
7. Oritz, L. et al., (1999) J Biol. Chem. 274, 15213-15221
8. Corthesy, B. et al., (1989) Mol. Cell. Biol. 9, 548-5562
9. Toohey, MG. et al., (1990) J Viol. 64, 4477-4488
10. Candeliere, AG. et al., (1996) Mol. Cell. Biol. 16, 584-592

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DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.