For protein expression and purification in E.coli, customer has five different tags for selecting: GST, MBP, His, Flag, biotin. Customer could choose N terminal or C terminal insert of the tag. We also provide tag remove and endotoxin removal services.
Cloning
Subcloning
Generation of bacmid DNA
Multiprotein Complex Expressions
Transfection
Tittering and amplification of virus
Optimization of the expression conditions
Mini- or large-scale infection
Conventional purification
Affinity purification
Dialysis in a proper buffer and measure of concentration
SDS-PAGE and Western blot analysis
Protocol optimization
Benefits
Time Savings: Reduce turn around time associated with pre-clinical development. Extend the capabilities of your research.
Excellence: Our protein expression and purification experience ensures product quality and accuracy.
Cost: Custom service, rapid turnaround at cost effective prices.
Protein One Capabilities
With protein production and purification capacities from 1mg to over 100mg, Protein One can accommodate projects ranging in size from bench top to bioreactor. From target discovery through pre-clinical development and beyond, Protein One is the best source for high quality protein production, outstanding service, and reputation that has made Protein One a market leader.
Please contact us for a quote or further information:
