E2F-1, RBAP-1, Transcription Factor and Rb-mediator

• Species; Human
• Expression Host: Baculovirus –insect cell
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 48.6 kDa.
• Gene Accession Number: NM_005225.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified E2F-1 is greater than 90% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays.

Applications

Recombinant E2F-1 protein can be used 1) for in vitro function studies including transcription, DNA binding and DNA bending assays; 2) for protein-protein interaction assay; and 3) for cell growth and proliferation assays.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

E2F-1; E2F1; RBAP1; RBBP3 and RBP3

Protein Sequence

ALAGAPAGGP CAPALEALLG AGALRLLDSS QIVIISAAQD ASAPPAPTGP AAPAAGPCDP
DLLLFATPQA PRPTPSAPRP ALGRPPVKRR LDLETDHQYL AESSGPARGR GRHPGKGVKS
PGEKSRYETS LNLTTKRFLE LLSHSADGVV DLNWAAEVLK VQKRRIYDIT NVLEGIQLIA
KKSKNHIQWL GSHTTVGVGG RLEGLTQDLR QLQESEQQLD HLMNICTTQL RLLSEDTDSQ
RLAYVTCQDL RSIADPAEQM VMVIKAPPET QLQAVDSSEN FQISLKSKQG PIDVFLCPEE
TVGGISPGKT PSQEVTSEEE NRATDSATIV SPPPSSPPSS LTTDPSQSLL SLEQEPLLSR
MGSLRAPVDE DRLSPLVAAD SLLEHVREDF SGLLPEEFIS LSPPHEALDY HFGLEEGEGI
RDLFDCDFGD LTPLDF

Background

E2F was originally identified as a transcription factor mediating the transcription of adenovirus E2 gene. It is a heterodimer composed of two structurally related subunits, termed E2F and DP. E2F is encoded by at least five genes, E2F-1 through E2F-5, and DP is encoded by at least two genes, DP-1 and DP-2. All these genes (E2F-1 through E2F-5, DP-1 and DP-2) have been considered as members of the E2F gene family because they have a number of common structural and functional characteristics (1-4). Among those members of the E2F family, E2F-1 was the first E2F gene cloned and characterized. The E2F-1 protein has been shown to interact with RB both in vitro and in vivo (5, 6). Although the E2F proteins can function as transcription factors by themselves, they should be considered as RB-mediators as well because almost every functional response of RB protein(s) requires the presence of E2F protein(s).

References

1. Bagchi, S. et al., (1990) Cell 62, 659-669.
2. Helin, K. et al., (1992) Cell 70, 337-350.
3. Kaelin, WG. et al., (1992) Cell 70, 351-364.
4. Shan, B. et al., (1992) Mol. Cell. Biol. 12, 5620-5631.
5. Chellappan, SP. et al., (1991) Cell 65, 1053-1061.
6. Schwarz, JK. et al., (1993) EMBO J. 12, 1013-1020.

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.