Purification and Quality Control The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified FGF-2 is greater than 95% homogeneous based on SDS-PAGE analysis.
Unit Definition (Activity) 1unit equals 1 nanogram of purified protein; 20 units are sufficient in cell culture experiments.
Applications FGF2 has been applied in cell culture experiments.
Formulation and Storage The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Background Acidic fibroblast growth factor (FGF-1) and basic fibroblast growth factor (FGF-2) are ubiquitous cytokines found in many tissues. They have effects on multiple cell types derived from mesoderm and neuroectoderm, including endothelial cells (1). FGF proteins are small peptides of 155 to 268 amino acid residues (2-4). The degree of sequence identity between different family members is 30-60% in a "central domain" of ~120 amino acids. This domain confers to FGFs a common tertiary structure and the ability to bind to heparin (5). Secreted FGFs signal to target cells by binding and activating cell-surface tyrosine kinase FGF receptors (FGFRs; 6, 7). The function of FGFs and FGFRs during embryonic development and adult physiology has been addressed by gain- and loss-of-function experiments in several animal model organisms. These studies have shown that FGFs act as key regulators of developmental events (8, 9).
Human FGF2 occurs in low molecular weight (LMW) and high molecular weight (HMW) isoforms. LMW FGF2 is primarily cytoplasmic and functions in an autocrine
manner, whereas HMW FGF2s are nuclear and exert activities through an intracrine
mechanism.Gene synonym include BFGF; FGFB and HBGF-2.
References: 1. Slavin J., (1995) Cell Biol Int. 5, 431-44. 2. Cuevas, P., et al., (1995) Neurol Res 17, 396-399 3. De Moerlooze, L., et al., (2000) Development 127, 483-492 4. Hebert, JM, et al., (1990) Dev Biol 138, 454-463 5. Faham, S., et al., (1996) Science 271, 1116-1120 6. Dionne, CA, et al., (1990) EMBO J 9, 2685-2692 7. Johnson, DE, and Williams LT (1993) Adv Cancer Res 60, 1-41 8. Arman, E, et al., (1998) Proc Natl Acad Sci USA 95, 5082-5087 9. Gage, FH. (2000) Science 287, 1433-1438
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
