Purification and Quality Control The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified FRA-1 is greater than 95% homogeneous based on SDS-PAGE analysis.
Unit Definition (Activity) 1 unit equals 1 nanogram of purified protein. 20-200ng is sufficient for an in vitro transcription assay and 100ng is sufficient for a protein-protein interaction assay.
Applications FRA-1 can be applied in in vitro transcription assays, DNA-protein and protein-protein interaction assays.
Formulation and Storage The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Background The fos-related antigen 1 (Fra-1, or FosL1: Fos-like antigen 1) is a member of the Fos onco-protein family and a component of AP-1 transcription factor (1). Similar to other members of the family, Fra-1 contains a leucine zipper domain and a highly conserved C-terminal region (2). Transcription of Fra-1 gene is regulated by multiple cis-elements and 12-O-tetradecanoylphorbol-13-acetate (TPA) (3). Fra-1 expression is associated with proliferation and invasiveness of breast cancer cells and other cancer cells (4,5).
References: 1. Cohen DR, et al., (1989) Genes Dev. Feb;3(2):173-84. 2. Matsui M, et al., (1990) Oncogene. Mar;5(3):249-55. 3. Adiseshaiah P, et al., (2003) J Biol Chem. Nov 28;278(48):47423-33 4. Belguise K, et al., (2005) Oncogene. Feb 17;24(8):1434-44. 5. Wang HL, et al., (2002) Int J Cancer. Oct 1;101(4):301-10.
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
