Farnesoid-X-activated Receptor -Rattus norvegicus

• Species: Rattus Norvegicus
• Expression Host: E.coli
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 56.6 kDa.
• Gene Accession Number: AAH83815.1.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified FXR is greater than 90% homogeneous based on SDS-PAGE analysis.

Applications

FXR has been applied in DNA and protein-protein interactions assays.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

Nr1h4 (Rattus norvegicus), FXR.

Protein Sequence

MVMQFQGLEN PIQISLQHSH RLSGFASDRM SSKPAKGVLT EHAAGPLGQN LDLESYSPYN
NVQFPQVQPQ ISSSSYYSNL GFYPQQPEDW YSPGLYELRR MPTESVYQGE TEVSEMPVTK
KPRMAASSAG RIKGDELCVV CGDRASGYHY NALTCEGCKG FFRRSITKNA VYKCKNGGNC
VMDMYMRRKC QDCRLRKCRE MGMLAECLLT EIQCKSKRLR KNVKQHADQT VNEDSEGRDL
RQVTSTTKLC REKTELTVDQ QTLLDYIMDS YSKQRMPQEI TNKILKEEFS AEENFLILTE
MATSHVQILV EFTKRLPGFQ TLDHEDQIAL LKGSAVEAMF LRSAEIFNKK LPAGHADLLE
ERIRKSGISD EYITPMFSFY KSVGELKMTQ EEYALLTAIV ILSPDRQYIK DREAVEKLQE
PLLDVLQKLC KIYQPENPQH FACLLGRLTE LRTFNHHHAE MLMSWRVNDH KFTPLLCEIW
DVQ

Background

Farnesoid-X-receptor (FXR) was originally identified and cloned in rat as an orphan nuclear hormone receptor based on hybridization with a degenerate oligonucleotide designed from the highly conserved nuclear hormone receptor DNA binding domain (1). FXR functions as a heterodimer with RXR and binds to sequence elements in the promoters of target genes. The FXR/RXR heterodimer binds with highest affinity to inverted repeats separated by 1 bp (IR-1) and with low affinity to direct repeats separated by 4 and 5 bp (DR-4 and DR-5) (1, 2). As is the case for other nuclear hormone receptors, FXR regulates target gene activity in response to ligand. While initial studies suggested that farnesol and retinoid metabolites were likely ligands for FXR, current data support the notion that FXR is a bile acid sensor that plays an integral role in bile acid synthesis and transport (1, 3-6). In the small intestine, FXR regulates bile acid uptake through the upregulation of the ileal bile acid binding protein gene via binding to an upstream response element (7). The FXR/RXR heterodimer can be activated by the bile salt chenodeoxycholic acid (CDCA) and FXR is required for the bile salt-dependent transcriptional control of the human ABCB11 gene (the bile salt export pump) (8). In addition, FXR has been shown to inhibit the cholesterol 7-hydrolase gene (CYP7A1) transcription (9).

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.