Glucokinase mediates phosphorylation of glucose. Allosteric agents which could activate GK activities or increase GK affinity to glucose will be a useful lead molecular to develop novel therapeutics for anti-diabetes. Proassay Glucokinase kit is a convenient, high-throughput method for enzyme-luminescence detection of glucokinase activity, which can be used for screening of glucokinase modulators.
Glucokinase (GK, hexokinae IV, D) mediates phosphorylation of glucose at the sixth carbon position in the initiation step of glycolysis and glycogen synthesis in the liver (1). GK plays central role in blood glucose homeostasis and has low affinity to glucose in physiological conditions. The increased glucose level induces the conformational change of GK from inactive form to active form to keep glucose homeostasis (2). Allosteric agents which could activate GK activities or increase GK affinity to glucose will be a useful lead molecules to develop novel therapeutics for anti-diabetes. Recently, several GK activating agents were reported as a candidates for anti-diabetic drugs (3-5). This user-friendly and highly sensitive glucokinase assay kit is a useful tools to explorer novel glucokinase modulators for anti-diabetes drug development. This luminescent system has several advantages such as non-radioactive and homogeneous, no requirement of specific antibodies, short hand-on times, cost-effective HTS assay system over other assay systems (6-8).
Kit components • Glucokinase (Liver type, ProteinOne, cat# P5052) • Assay buffer • Control GK activator • Detection reagent • White microplate • User manual
System developed for screening of Glucokinase modulators (activators) and glucokinase related assays. Extensive quality control has been conducted. P9007-01 (100 assays / 96-well format) P9007-02 (200 assays / 96-well format) Please see description for details.
References: 1. Kietzmann T and GK Ganjam 2005. Expert Opin Ther Patents 15:705-713 2. Brocklehurst et al., 2004. Diabetes 53:535-540 3. Grimsby et al., 2003. Science 301:370-373 4. Futamura et al., 2006. J. Biol. Chem. 281:37668-37674 5. Fyfe et al., 2007. Diabetologia 50:1277-1287 6. Baki A etal., 2007. Assay Drug Dev Technol 5:75-83 7. Koresawa, T and Okabe T. 2004. Assay 2:153-160 8. Klumpp, M etal., 2006. J. Biomol Screen 11:617-633
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
