His-tagged full length PPAR-alpha(cat# p1048-01, 10ug)
His-tagged ligand binding domain of PPAR-alpha (p1063-01, 10ug)
GST tagged full length PPAR-alpha (p4030-01, 10ug)
GST tagged ligand binding domain of PPAR-alpha(p4031-01, 10ug)
There is evidence that a group of closely related nuclear receptors, called peroxisome proliferator-activated receptors (PPARs), may be involved in chronic diseases such as diabetes, obesity, artherosclerosis and cancer. The PPARs were first cloned as the nuclear receptors that mediate the effects of synthetic compounds called peroxisome proliferators on gene transcription. It soon became clear that eicosanoids and fatty acids can also regulate gene transcription through PPARs. They bind a specific element in the promoter region of target genes only as a heterodimer with the receptor for 9- cis retinoic acid, RXR (retinoid X receptor). Binding of the ligand of either receptor can activate the complex, but binding of both ligands simultaneously is more potent (1). Three PPAR isotypes have been identified: α, β (also called NUC1) and γ. PPARα is expressed most in brown adipose tissue and liver, then kidney, heart and skeletal muscle. PPARβ is found in many tissues but the highest expression is in the gut, kidney and heart. PPARγ is mainly expressed in adipose tissue, and to a lesser extent in colon, the immune system and the retina (2). The target genes of PPARα are a relatively homogenous group of genes that participate in aspects of lipid catabolism such as fatty acid uptake through membranes, fatty acid binding in cells, fatty acid oxidation (in microsomes, peroxisomes and mitochondria) and lipoprotein assembly and transport (3).
Recombinant His-tagged and GST-tagged PPARα; His-tagged and GST-tagged ligand binding domain of PPAR-alpha were expressed and purified from E. coli system.
PPARα has been applied in DNA and protein-protein interaction assays. Purified protein is greater than 95% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 20 units are sufficient for a gel-mobility shift assay and 100 units are sufficient for a protein-protein interaction assay.
0.5 mg/ml (in 1X dilution buffer A) 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
