Retinoic acid receptors are important in the regulation of growth and differentiation of epithelial tissues, embryonic and central nervous system development and hematopoiesis (1). Retinoids mediate their effect by two classes of nuclear receptor proteins, the retinoic acid receptors (RARs) and the retinoid X receptors (RXRs), that each consist of three isotypes (α, β, and γ) encoded in separate genes (2). Upon dimerization with RXR, RARs can bind to specific enhancer sequences in the DNA, so-called retinoic acid response elements (RAREs), resulting in transcriptional activation of target genes in the presence of ligand (3). Retinoids, the natural and synthetic vitamin A derivatives, are known to inhibit the proliferation of lung cancer and breast cancer cells and the growth of carcinogen-induced bronchogenic squamous cell carcinoma and mammary tumors, and have been used as chemoprevention agents against both types of cancer (4). A growing body of evidence supports the hypotheses that the RAR beta gene is a tumor suppressor gene and the chemopreventive effects of retinoids are due to induction of RAR beta. RAR beta expression is reduced in many malignant tumors including breast carcinoma. (5).

Recombinant His tagged RAR beta was expressed in a baculovirus system; His-tagged and GST tagged ligand binding domain of RAR beta were expressed from E.coli system.

RAR beta has been applied in in vitro transcription assays, DNA and protein-protein interaction assays.

The purified recombinant protein is greater than 90% homogeneous based on SDS-PAGE analysis.
1 unit is equal to 1 nanogram of purified protein. 20-100 units are sufficient for an in vitro transcription assay and 100 units are sufficient for a protein-protein interactions assay.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA

References:
1. Kastner et al. (1995) Cell, 83, 859-869
2. Leid et al., (1992) Trends Biochem. Sci. 17, 427-433
3. Linney et al., (1992) Curr. Top. Dev. Biol. 27, 309-350
4. Zhang et al., (1996) Mutat. Res. 350, 267-277
5. Yang et al., (2002) Breast Cancer Res. Treat. 76, 167-173

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This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.