GST-tagged activating transcription factor 2 (19-96)

• Species: Human
• Expression: Host E.coli
• Tag: GST-tag
• Purity: 90%
• Molecular Weight: 37.1 kDa.
• Gene Accession Number: BC130335.

The activating transcription factor 2 (ATF2), known as CRE-BP1 (cAMP-responsive element binding protein 1), is a member of the leucine zipper family of DNA binding proteins and binds to both AP-1 and CRE DNA response elements of many viral and cellular promoters (1). ATF2 is activated by diverse cellular stresses such as inflammatory cytokines, genotoxic agents and UV irradiation. It is activated through phosphorylation at two phospho sites of Thr69 and Thr71 by upstream activators like JNK/p38 (MAPK14, SAPK2A) (2-4). GST-ATF2 [19-96] is preferentially used as a useful substrate for MAPK14 and was assayed using MAPK14 (P5049 and R5048) by luminescence-based ATP depletion assay.

Synonyms
Gene synonym include CRE-BP1; HB16 and TREB7.

Target gene sequence

Formulation and Storage
Recombinant GST-ATF2 [19-96] is isolated from an E coli strain that carries the coding sequence of the human ATF2 [19-96] under the control of a T7 promoter.

Application
GST can be used for a protein-protein interaction assay and a protein-DNA interaction assay (6, 7). 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay. For Research use only.

Image of SDS-PAGE