The soluble Glutathione S-Transferase is a 27 kDa protein which occurs as a dimer in all aerobic organisms. Each monomer has two domains, one that binds GSH and is an α/β-structure similar to thioredoxin and the other, all helical, that binds the hydrophobic substrate (1). The GST-fusion protein expression system is a widely used recombinant protein expression system that allows a peptide or a regulatory protein domain to be expressed as a fusion to the C-terminus of Schistosoma japonicum GST (2). Fusion proteins also possess GST-enzymatic activity and can undergo dimerization similar to in vivo. The fusion protein can be purified via GST-affinity column chromatography. In most cases, the desired peptides or domains are removed from GST by applying a specific protease that recognizes and cleaves the linker between the protein domain and GST. The technique has been widely used to generate different kinds of proteins for crystallization (3), molecular immunology studies (4), the production of vaccines (5) and studies involving protein-protein (6) and protein-DNA interactions (7).

Isolated from an E. coli strain that carries the coding sequence for Schistosoma japonicum GST under the control of a T7 promoter (2). Gene access number is: AAA57089.

GST can be used for a protein-protein interaction assay and a protein-DNA interaction assay (6, 7).
The purified recombinant protein is greater than 95% homogeneous based on SDS-PAGE analysis.

Anti-GST pAb was produced from rabbit. Each vial contains 100 μg IgG in PBS.

Specificity:
α-GST reacts with the GST protein in HeLa nuclear extract by Western Blotting.

Storage
Aliquot and store at –20°C.

1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT, and 0.2 mM EDTA

References:
1.Ketterer, B. (2001) Chem Biol Interact138(1):27-42
2. Smith, D.B. et al., (1988) Gene 67, 31-34
3. Zan et al., (2001) Gene 281(1-2):1-9
4. Toye, B. et al., (1990) Infect. Immun. 58, 3909-3914
5. Fikrig, E., et al., (1990) Science 250, 553-556
6. Kaelin, W.G. et al., (1991) Cell 64, 521-529
7. Kaelin, W.G. et al., (1991) Cell 65, 1073-1080

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.