Retinoic acid receptors are important in the regulation of growth and differentiation of epithelial tissues, embryonic and central nervous system development and hematopoiesis (1). Retinoids mediate their effect by two classes of nuclear receptor proteins, the retinoic acid receptors (RARs) and the retinoid X receptors (RXRs), that each consist of three isotypes (α, β, and γ) encoded in separate genes (2). Upon dimerization with RXR, RARs can bind to specific enhancer sequences in the DNA, so-called retinoic acid response elements (RAREs), resulting in transcriptional activation of target genes in the presence of ligand (3). The RAR-gamma in the adult is found almost exclusively in the skin (4). Retinoids affect epidermal cell growth and differentiation as well as sebaceous gland activity and exhibit immunomodulatory and anti-inflammatory properties (5). Current retinoid research targets the development of receptor-selective retinoids for tailoring and/or improving their therapeutic profile (6).

Synonyms: NR1B3; RARG and RARC.
GST-RARγ was expressed in an E.coli system and purified by a combination of affinity and gel filtration chromatography.
RARγ has been applied in in vitro transcription assays and in DNA and protein-protein interaction assays.
The purified recombinant protein is greater than 90% homogeneous based on SDS-PAGE analysis.
1 unit equals 1 nanogram of purified protein. 20 ng is sufficient for an in vitro transcription assay and 100 ng is sufficient for a protein-protein interaction assay.
variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA

References:
1. Kastner et al. (1995) Cell, 83, 859-869
2. Leid et al., (1992) Trends Biochem. Sci. 17, 427-433
3. Linney et al., (1992) Curr. Top. Dev. Biol. 27, 309-350
4. Rees J. (1992) Br. J. Dermatology 126, 97-104
5. Orphanos et al., (1997) Drugs 53, 358-388
6. Zhao et al., (2003) J. Biomed. Sci. 10, 44-49

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This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.