The TATA-binding protein (TBP) is believed to function as an essential factor of the general transcription machinery and to be involved in transcription by all three eukaryotic RNA polymerases (pol I, II, and III). TBP specifically binds to the TATA element at the promoter region and interacts with numerous transcription factors, including TBP-associated factors (TAFs), activators, and some tumor suppressor proteins (1-3).
Isolated from an E. coli strain that carries the coding sequence for human TBP under the control of T7 promoter (4). GST-TBP can be used for protein-protein interaction assay (5).
The purified protein is greater than 95% homogeneous based on SDS-PAGE analysis. 1 unit equals 1 nanogram of purified protein. 100 units are sufficient for a protein-protein interaction assay detected by an immuno-blot system.
variable in different lots 1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA
References: 1. Horikoshi, M. et al., (1988) Cell 54, 1033-1042 2. Hernandez, N. (1993) Genes & Dev. 7, 1291-1308 3. Holffman, A. et al., (1991) Nucleic Acids Res. 19, 6337-6338 4. Ge, H. et al., (1994) J. Biol. Chem. 269, 17136-17140 5. Kaludov, N., et al., (2000) Nucleic Acids Res. 28, 1921-1928
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