HMG1 Package Package contains

• native HMG1 (Cat# p1058-01, 10ug)
• His-tagged recombinant
• low endotoxin HMG1 (Cat# p1025-01, 10ug)
• anti-HMG1 pAb (Cat# PA112-01, 250ul)

High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein (1). Mammalian HMG1 has two homologous DNA-binding domains: HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues (2). HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compacting or unwinding, or by direct contacts with distinct cellular proteins (3-5). HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation (6, 7) or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences (8, 9). Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release (10).

Recombinant His tagged HMG1 is isolated from an E. coli strain that carries the coding sequence of the human HMG1 under the control of a T7 promoter.

HMG1 can be applied in in vitro transcription assays, DNA-protein and protein-protein interactions assays.
The protein is greater than 95% homogeneous based on SDS-PAGE analysis. Low endotoxin levels, 1 unit equals 1 nanogram of purified protein. 1 unit is the amount sufficient for a gel mobility shift assay in a 20 µl reaction to super-shift TBP-DNA complex, 20 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.

variable in different lots
1x dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA

α-HMG-1 is rabbit polyclonal antibody raised against the recombinant full-length HMG-1 protein.

Each vial contains 0.25 ml of anti-serum.

Specificity
α-HMG-1 reacts with the HMG-1 protein in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:2000.

Storage

Aliquot and store at –20°C.