High Mobility Group 1 protein (Low Endotoxin)

• Species; Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 95%
• Molecular Weight: 27.3 kDa.
• Gene Accession Number: NM_002128.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified HMG1 is greater than 95% homogeneous based on SDS-PAGE analysis.

Endotoxin is removed during chromatography purification process. Low endotoxin levels,

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays.

Applications

HMG1 can be applied in in vitro transcription assays, DNA-protein and protein-protein interactions assays.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

HMG1; HMG3; SBP-1.

Protein Sequence

GKGDPKKPRG KMSSYAFFVQ TCREEHKKKH PDASVNFSEF SKKCSERWKT MSAKEKGKFE DMAKADKARY EREMKTYIPP KGETKKKFKD PNAPKRPPSA FFLFCSEYRP KIKGEHPGLS IGDVAKKLGE MWNNTAADDK QPYEKKAAKL KEKYEKDIAA YRAKGKPDAA KKGVVKAEKS KKKKEEEEDE EDEEDEEEEE DEEDEDEEED DDDE

Background

High mobility group 1 (HMG1) is a 26 kDa highly conserved non-sequence-specific DNA-binding nuclear protein (1). Mammalian HMG1 has two homologous DNA-binding domains: HMG boxes, A and B (each of 80–90 amino-acid residues), linked by a short basic region to an acidic C-terminal domain containing 30 consecutive Asp and Glu residues (2). HMG1 has been implicated in a number of fundamental biological processes including transcription, replication and recombination, in which it plays a role in manipulating DNA structure by bending, looping, compacting or unwinding, or by direct contacts with distinct cellular proteins (3-5). HMG-1 can act as a repressor, by interacting with TBP to block pre-initiation complex formation (6, 7) or as an activator, by facilitating the binding of various transcription factors to their cognate DNA sequences (8, 9). Most recently, it was discovered that HMG-1 is a late mediator of delayed endotoxin lethality by activating downstream cytokine release (10).
Recombinant His tagged HMG1 is isolated from an E. coli strain that carries the coding sequence of the human HMG1 under the control of a T7 promoter.

Image of SDS-PAGE / Western-blot

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.