Recombinant mouse IFN lambda/IL28B (His and MBP tagged) was purified from a bacterial system. IFN lambda gene access number is NM_177396.1. M21001 contains 20ug.

IL28B/IFN-lambda belongs to type III interferons that are class II cytokine receptor ligands (1). Expression of these cytokines can be induced by viral infection, such as influenza A. Lambda interferon renders epithelial cells of the respiratory and gastrointestinal tracts resistant to viral infections (6). Beside antiviral activity, mouse IFN-lambda also exhibit antitumor activity against B16 melanoma (7).

Interleukin 28B gene encodes a cytokine distantly related to type I interferons and the IL-10 family. Interleukin 28A (IL28A), IL28B and interleukin 29 (IL29) are three closely related cytokine genes that form a cytokine gene cluster on a chromosomal region mapped to 19q13 (1, 2). These cytokines exert bioactivities that overlap those of type I IFNs, including antiviral activity and up-regulation of MHC class I antigen expression (2, 3). IL28B signals through a heterodimeric receptor complex that is composed of the IL-10 receptor beta (IL-10 R beta) and a novel IL-28 receptor alpha (IL-28 R alpha, also known as IFN-lambda R1). Binding to its receptor activated JAK tyrosine kinase, then leads to STAT phosphorylation and the formation of the IFN-stimulated regulatory factor 3(ISGF-3) transcription factor complex (1, 4). Mouse IL-28B cDNA encodes a 193 amino acid (aa) protein with a 19 aa signal peptide and a 174 aa mature protein that lacks N-glycosylation sites. Mature mouse IL-28B shares 64% aa sequence identity with human (5).

Source:
Recombinant mouse IL28B/IFN lambda has the matured 174 aa at its C terminal, his tag and MBP tag at the N terminal. It is expressed and purified from an E.coli system. Its full molecular weight is 63.3 kD.

Applications:
IL28B has been applied in anti-viral assay. For research use only.

Quality Control:
Protein is greater than 90% homogeneous based on SDS-PAGE analysis.

Concentration:
0.5 mg/ml (in 1x dilution buffer A)

Reagents Supplied:
Dilution buffer A: 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA.

Reference:
1. Chen, Q. et al (2006) Vitam. Horm 74:207.
2. Kotenko, S.V. et al (2003) Nat. Immunol. 4:69.
3. Onoguchi, K. et al. (2007) J. Biol. Chem. 282:7576.
4. Sheppard, P. et al (2003) Nat. immunol. 4: 63.
5. Ank, N. et al. (2006) J. Virol. 80:4501.
6. Mordstein, M. et al (2010) J. Virol. 84 (11), 5670-5677.
7. Lasfar, A. et al(2006) Cancer Res. 66 (8), 4468-4477

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.