Purification and Quality Control
The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified KLF4 is greater than 90% homogeneous based on SDS-PAGE analysis.

Applications
May be used in stem cell related research. For Research use only.

Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Protein Sequence
MRQPPGESDM AVSDALLPSF STFASGPAGR EKTLRQAGAP NNRWREELSH MKRLPPVLPG RPYDLAAATV ATDLESGGAG AACGGSNLAP LPRRETEEFN DLLDLDFILS NSLTHPPESV AATVSSSASA SSSSSPSSSG PASAPSTCSF TYPIRAGNDP GVAPGGTGGG LLYGRESAPP PTAPFNLADI NDVSPSGGFV AELLRPELDP VYIPPQQPQP PGGGLMGKFV LKASLSAPGS EYGSPSVISV SKGSPDGSHP VVVAPYNGGP PRTCPKIKQE AVSSCTHLGA GPPLSNGHRP AAHDFPLGRQ LPSRTTPTLG LEEVLSSRDC HPALPLPPGF HPHPGPNYPS FLPDQMQPQV PPLHYQELMP PGSCMPEEPK PKRGRRSWPR KRTATHTCDY AGCGKTYTKS SHLKAHLRTH TGEKPYHCDW DGCGWKFARS DELTRHYRKH TGHRPFQCQK CDRAFSRSDH LALHMKRHF

Background
In embryonic stem cells (ESCs), KLF4 has been demonstrated to be a good indicator of stem-like capacity. As a transcription factor, it can act both as activator and as repressor. KLF4 binds the 5'-CACCC-3' core sequence and regulates the expression of key transcription factors during embryonic development. It plays an important role in maintaining embryonic stem cells, and in preventing their differentiation. KLF4 also contributes to the down-regulation of p53/TP53 transcription.

References
1, Takahashi K., Tanabe K., et al. (2007) Cell 131:861-872.
2, Zhang P., Andrianakos R., et al. (2010) J. Biol. Chem. 285:9180-9189.
3,Rowland B., Bernards R., et al. (2005) Nature Cell Biology 7, 1074 - 1082.

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.