Purification and Quality Control
The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified MAPK14 is greater than 90% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)
1 unit equals 1 nanogram of purified protein. 100ng is sufficient for a protein-protein interaction assay.

Applications
100ng of MAPK14 is sufficient to phosphorylate 1 ug of heat-inactivated nuclear or cytoplasmic extract at 30 C for 30 mins.

Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym
CSBP1; CSBP2; CSPB1; EXIP; Mxi2; p38; p38ALPHA; PRKM14; PRKM15; RK and SAPK2A.

Protein Sequence
MSQERPTFYR QELNKTIWEV PERYQNLSPV GSGAYGSVCA AFDTKTGLRV AVKKLSRPFQ
SIIHAKRTYR ELRLLKHMKH ENVIGLLDVF TPARSLEEFN DVYLVTHLMG ADLNNIVKCQ
KLTDDHVQFL IYQILRGLKY IHSADIIHRD LKPSNLAVNE DCELKILDFG LARHTDDEMT
GYVATRWYRA PEIMLNWMHY NQTVDIWSVG CIMAELLTGR TLFPGTDHIN QLQQIMRLTG
TPPAYLINRM PSHEARNYIQ SLTQMPKMNF ANVFIGANPL AVDLLEKMLV LDSDKRITAA
QALAHAYFAQ YHDPDDEPVA DPYDQSFESR DLLIDEWKSL TYDEVISFVP PPLDQEEMES

Background
Production of interleukin-1 and tumor necrosis factor (TNF) are inhibited by compounds called CSAIDs (cytokine-suppressive antiinflammatory drugs). 2 CSBPs (CSAID-binding proteins) have been characterized as molecular targets of pyridinyl-imidazole cytokine inhibitors. The 2 closely related targets are mitogen-activated protein kinases (MAPKs) CSBP1 and CSBP2. (1) MAPK14 is activated in response to many cellular stresses and also regulates the differentiation and/or survival of various cell types in vitro, including skeletal muscle cells and cardiomyocytes. Studies indicate that MAPK14 is required for placental organogenesis but is not essential for other aspects of mammalian embryonic development. (2)The inhibition of p38alpha MAPK blocks the release of vascular endothelial growth factor and suppresses tumor-promoted endothelial cell migration, a key step in angiogenesis.(3)

References
1. Lee, J. C.; et al., Nature 372: 739-746, 1994.
2. Adams, R. H et al., Molec. Cell 6: 109-116, 2000.
3. Hendrickx,N.,et al., Biochem. Biophys. Res. Commun. 337 (3), 928-935 (2005)

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.