Purification and Quality Control This His tagged recombinant MDM2 was expressed in an E. coli system and purified by affinity and FPLC chromatography. The purified recombinant protein is greater than 95% homogeneous based on SDS-PAGE analysis.
Unit Definition (Activity) 1 unit equals 1 nanogram of purified protein. Variation exist in different lots.
Applications MDM2 can be applied in in vitro transcription assays, DNA-protein and protein-protein interaction assays.
Formulation and Storage MDM2 is stored in 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA buffer. MDM2 is easy degraded. Please keep in -80°C and avoid freeze thaw cycle.
Synonym Homo sapiens Mdm2 p53 binding protein homolog (mouse) (MDM2); hdm2; HDMX; MGC5370 and MGC71221.
Background Originally discovered as one of three genes amplified on double minute chromosomes in a tumorigenic derivative of NIH 3T3 cells (1), MDM2 was later shown to possess oncogenic potential when overexpressed (2, 3). High-level expression of MDM2 has also been shown to confer tumorigenic potential upon nontransformed rodent fibroblasts in athymic nude mice (2, 3). MDM2 can immortalize rat embryo fibroblasts and can cooperate with activated RAS to transform these cells (3). Elevated levels of MDM2 protein have been found in a variety of human tumors, most notably in soft tissue sarcomas where up to 30% of primary tumors contain multiple copies of the MDM2 gene (4). One mechanism by which MDM2 overexpression promotes tumor development is through its ability to bind to the p53 tumor suppressor, thereby blocking the transactivation (5-7), cell cycle arrest (8), and apoptotic functions of p53 (9). MDM2 can inhibit p53 activity in a number of ways including preventing p53 from recruiting TAFs, promoting nuclear export, inhibiting p53 acetylation, and perhaps most importantly by virtue of its function as an E3 ubiquitin ligase with specificity for, among others, p53 (reviewed in 10). In addition to regulating p53 levels by targeting p53 for proteasomal degradation MDM2 also transfers ubiquitin to itself, MDMX, the ß2 adrenergic receptor, glucocorticoid receptor, TIP60, and PCAF (10).
References: 1. Cahilly-Snyder, L., et al., (1987) Somat. Cell Mol. Genet. 13:235-244. 2. Fakharzadeh, S. et al., (1991) EMBO J. 10:1565-1569 3. Finlay, C. A., (1993) Mol. Cell. Biol. 13:301-306 4. Leach, F. S., et al., (1993) Cancer Res. 53:2231-2234. 5. Momand, J., et al., (1992) Cell 69:1237-1245. 6. Oliner, J. D., et al., (1992) Nature 358:80-83 7. Oliner, J. D., et al., (1993) Nature 362:857-860 8. Chen, J., et al., (1996) Mol. Cell. Biol. 16:2445-2452. 9. Dornan, D., et al., (2004) Nature 429:86-92 10. Bond GL, et al., (2005) Current Cancer Drug Targets. 5 (1):3-8.
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
