Nuclear factor (erythroid-derived 2)-like 2, also known as NFE2L2 or Nrf2

• Species; Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 95%
• Molecular Weight: 69.9 kDa.
• Gene Accession Number: BC011558.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified Nrf2 is greater than 95% homogeneous based on SDS-PAGE analysis.

Applications

Recombinant Nrf2 can be used for DNA-protein, protein-protein interaction research and assay development.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

Nuclear factor (erythroid-derived 2)-like 2, NFE2L2,

Protein Sequence

MMDLELPPPG LPSQQDMDLI DILWRQDIDL GVSREVFDFS QRRKEYELEK QKKLEKERQE
QLQKEQEKAF FAQLQLDEET GEFLPIQPAQ HIQSETSGSA NYSQVAHIPK SDALYFDDCM
QLLAQTFPFV DDNEVSSATF QSLVPDIPGH IESPVFIATN QAQSPETSVA QVAPVDLDGM
QQDIEQVWEE LLSIPELQCL NIENDKLVET TMVPSPEAKL TEVDNYHFYS SIPSMEKEVG
NCSPHFLNAF EDSFSSILST EDPNQLTVNS LNSDATVNTD FGDEFYSAFI AEPSISNSMP
SPATLSHSLS ELLNGPIDVS DLSLCKAFNQ NHPESTAEFN DSDSGISLNT SPSVASPEHS
VESSSYGDTL LGLSDSEVEE LDSAPGSVKQ NGPKTPVHSS GDMVQPLSPS QGQSTHVHDA
QCENTPEKEL PVSPGHRKTP FTKDKHSSRL EAHLTRDELR AKALHIPFPV EKIINLPVVD
FNEMMSKEQF NEAQLALIRD IRRRGKNKVA AQNCRKRKLE NIVELEQDLD HLKDEKEKLL
KEKGENDKSL HLLKKQLSTL YLEVFSMLRD EDGKPYSPSE YSLQQTRDGN VFLVPKSKKP
DVKKN

Background

Nuclear factor (erythroid-derived 2)-like 2, also known as NFE2L2 or Nrf2, is a transcription factor that binds to the antioxidant response element (ARE) of target genes in response to oxidative stress and increases the transcription of a variety of antioxidative and carcinogen-detoxification enzymes. Stress can result from a variety of causes, including fasting, overfeeding, endogenous compounds, exposure to chemicals, or environmental agents, which generally leads to increased production of reactive oxygen species (ROS). Under normal conditions, Nrf2 is bound to Kelch like-ECH-associated protein 1 (Keap1). Keap1 acts as a substrate adaptor protein for Cullin 3-based ubiquitination, which results in the degradation of Nrf2, and under normal conditions Nrf2 has a half-life of only 20 minutes. Oxidative stress or electrophilic stress disrupts critical cysteine residues in Keap1, resulting in a disruption of the Keap1-Cul3 ubiquitination system and a build-up of Nrf2 in the cytoplasm and translocates to the nucleus, where it binds to AREs to induce expression of multiple cytoprotective enzymes, including NAD(P)H-quinone oxidoreductase 1 (NQO1), glutathione S-transferases (GSTs), and heme oxygenase 1 (HO-1) ( 1 , 2 ).

Nrf2 also induces several transcription factors involved in mitochondrial biogenesis. Many lines of evidence suggest that mitochondrial dysfunction plays a central role in the pathogenesis of PD. Thus, Nrf2 may be a promising target to counteract mitochondrial dysfunction and its consequences in Parkinson's disease (PD) (3). The Keap1–Nrf2 regulatory system is a double-edged sword for our body. High-level Nrf2 activity increases cell protection and makes the cell resistant to oxidative and electrophilic stresses. Therefore, Nrf2 inducers could be effective for cancer chemoprevention. On the other hand, elevated Nrf2 activity is also advantageous for cancer cell survival and evolution. Thus, Nrf2 inhibitors could be effective for anticancer therapy. (4)

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.