E1A binding protein p300, Tumor Suppressor Protein and Transcription Factor

• Species: Human
• Expression Host: Baculovirus-insect cell
• Tag: Flag-tag
• Purity: SDS-PAGE 90%
• Molecular Weight: 265.2 kDa
• Gene Accession Number: NM_001429.

Purification and Quality Control

The Flag-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified p300 is greater than 90% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 50 units are sufficient for reconstituted transcription assay and 100 units are sufficient for a protein-protein interaction assay.

Applications

Recombinant p300 can be used for: 1) protein-protein interaction assay; 2) in vitro transcription assay; 3) in vitro acetylation assay; and 4) cell growth assay.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

Homo sapiens E1A binding protein p300 (EP300), KAT3B; p300 and RSTS2.

Protein Sequence

Background

Human p300 and CBP (CREB binding protein) are highly related transcriptional coactivators. Both proteins have been identified through protein interaction assays (1-3). In addition to interacting with a variety of cellular factors and onco-proteins, loss of the wild type CBP alleles in isolated tumors suggests that CBP/p300 might serve as tumor suppressors (4). The ability of p300 to acetylate many transcription factors, including p53, E2F, TFIIE, and TFIIF etc. demonstrates a novel mechanism of targeted p300 regulation of gene expression (5-7).

Image of SDS-PAGE /Western-blot