p52, PC4 and SFRS1 interacting protein 1

• Species; Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 39.2 kDa.
• Gene Accession Number: NM_021144.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified P52 is greater than 95% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays.

Applications

Recombinant p52 can be utilized for in vitro function studies, including transcription, splicing, protein-DNA/RNA and protein-protein interactions.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

Homo sapiens PC4 and SFRS1 interacting protein 1 (PSIP1), transcript variant 1; DFS70; LEDGF; MGC74712; p52; p75; PAIP and PSIP2.

Protein Sequence

DFKPGDLIFA KMKGYPHWPA RVDEVPDGAV KPPTNKLPIF FFGTHETAFL GPKDIFPYSE NKEKYGKPNK RKGFNEGLWE IDNNPKVKFS SQQAATKQSN ASSDVEVEEK ETSVSKEDTD HEEKASNEDV TKAVDITTPK AARRGRKRKA EKQVETEEAG VVTTATASVN LKVSPKRGRP AATEVKIPKP RGRPKMVKQP CPSESDIITE EDKSKKKGQE GKQPKKQPKK DEEGQKEEDK PRKEPDKKEG KKEVESKRKN LAKTGVTSTS DSEEEGDDQE GEKKRKGGRN FQTAHRRNML KGQHEKEAAD RKRKQEEQME TEHQTTCNLQ

Background

The human p52 protein is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II. The function of p52 is through interactions with transcriptional activators and the basal transcription machinery (1). In addition, p52 may also interact with several cellular proteins including the transcription coactivator PC4, the essential splicing factor ASF/SF2 and the nuclear protein nucleolin (2, 3).

References

1. Ge, H. et al., (1998) EMBO J. 17, 6723-6729
2. Ge, H. et al., (1998) Molecular Cell 2, 751-759
3. Ge, H. (2000) Nucleic Acid Res. 28, e3

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.