p53 was identified as a tumor suppressor by blocking the transformation and inhibiting tumor cell growth. In addition, this protein is a transcription factor capable of regulating the expression of a subset of downstream genes (1-5). Different mutations in p53 gene have been characterized in a variety of human cancers (6, 7). Loss or mutation of p53 function is highly correlated with tumorigenesis.

Each vial contains 100 μg IgG in 0.1 ml of PBS.

Specificity
α-p53 reacts with the p53 protein in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:2000.

Storage
Aliquot and store at –20°C.

References:
1. Finlay, C. et al., (1989) Cell 57, 1083-1093
2. Michalovitz, D. et al., (1990) Cell 62, 671-680
3. Baker, S. et al., (1990) Science 249, 912-915
4. Fields, S. et.al., (1990) Science 249, 1046-1049
5. Raycroft, L. et al., (1990) Science 249, 1049-1051
6. Hollstein, M. et al., (1991) Science 253, 49-53
7. Bennett, W. et al., (1992) Chest 101, 19S-20S

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.