The human p75 protein (also called Lens Epithelium-derived Growth Factor: LEDGF), similar to p52, is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators (1, 2). Although p75 and p52 are derived from alternatively splicing of a single gene and share most coding sequence, they reveal different function in several aspects. In addition to functioning as a transcription coactivator, p75 has been reported to be involved in regulating growth of epithelial cells, pathogenesis of atopic dermatitis and activity of HIV-1 integrase (3-7).

Each vial contains 0.2 ml of anti-serum.

Specificity
α-p75 reacts specifically with p75 (LEDGF) protein (but not p52) in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:1000-1:5000.Recommended starting dilution: 1:2000.

Storage
Aliquot and store at -20°C.

References:
1. Ge, H. et al., (1998) EMBO J. 17, 6723-6729
2. Ge, H. et al., (1998) Molecular Cell 2, 751-759
3. Singh, DP. et al., (2000) Gene 242, 265-273
4. Singh, DP. et al., (1999) Invest Ophthalmol Vis. Sci. 40, 1444-1451
5. Ochs, RL. et al., (2000) J. Allergy Clin. Immunol. 105, 1211-1220
6. Wu, X. et al., (2002) Cell Death and Differentiation 9, 915-925
7. Cherepanov, P. et al., (2003) J. Biol. Chem. 28, 372-381

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.