The human PC4 (also called p15) is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators. The function of PC4 is apparently through interactions with transcriptional activators and the basal transcription machinery. It is negatively regulated by casein kinase II phosphorylation both in vivo and in vitro (1-4). PC4 strongly binds single stranded DNA and regulates HSSB (RPA)-dependent SV40 DNA replication (5, 6). Recent studies indicated that PC4 can be acetylated by several histone acetyltransferase (7).

Specificity
α-PC4 reacts with the PC4 protein in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:2000.

Storage
Aliquot and store at –20°C.

References:
1. Ge, H. et al., (1994) Cell 78, 513-523
2. Ge, H. et al., (1994) Proc. Natl. Acad. Sci. USA 91, 12691-12695
3. Kretzschmar, M. et al., (1994) Cell 78, 525-534
4. Wu, SY. et al., (1998) J. Biol. Chem. 273, 12492-12498
5. Brandsen, J. et al., (1997) Nat. Struct. Biol. 4, 900-903
6. Werten, S. et al., (1998) EMBO J. 17, 5103-5111
7. Ge, H. (2000) Nucleic Acids Res. 28,

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.