PC4, Positive Cofactor 4 wild type Transcriptional Coactivator
Species; Human
Expression Host: E.coli
Tag: His-tag
Purity: 95%
Molecular Weight: 14.4 kDa.
Gene Accession Number: NM_006713.
Purification and Quality Control
The recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified PC4 is greater than 90% homogeneous based on SDS-PAGE analysis. Protein is greater than 95% homogeneous and contains no detectable protease, DNase and RNase activity.
Unit Definition (Activity)
1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays.
Applications
Recombinant PC4 has been utilized for in vitro function studies, including transcription, DNA replication, in vitro phosphorylation, gel mobility shift assay, protein-protein interactions and as a substrate for in vitro acetylation.
Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Synonym
Homo sapiens SUB1 homolog (S. cerevisiae) (SUB1); MGC102747; p14 and P15.
The human PC4 is a non-TAF transcription coactivator that mediates activator-dependent transcription by RNA polymerase II in vitro through most tested activators. The function of PC4 is apparently through interactions with transcriptional activators and the basal transcription machinery. It is negatively regulated by casein kinase II phosphorylation both in vitro and in vivo (1-3). PC4 strongly binds single stranded DNA and regulates HSSB (RPA)-dependent SV40 DNA replication (4, 5). Recent studies indicated that PC4 can be acetylated by several histone acetyltransferases (6).
References
1. Ge, H. et al., (1994) Cell 78, 513-523; (1994) Proc. Natl. Acad. Sci. USA 91, 12691-12695 2. Kretzschmar, M. et al., (1994) Cell 78, 525-534 3. Ge, H. et al., (1996) Methods Enzymol. 274, 57-71 4. Ballard, DW. et al., (1988) J. Biol. Chem. 263, 8450-8457 5. Pan, ZQ. et al., (1996) J. Biol. Chem. 271, 22111-22116
6. Kumar, BR. et al., (2001) J. Biol. Chem. 276, 16804-1689
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
