PKACA (Protein Kinase A Catalytic subunit alpha)

• Species: Human
• Expression Host: E.coli
• Tag: No tag
• Purity: 95%
• Molecular Weight: 40.5 kDa.
• Gene Accession Number: NM_002730.

Applications
PKA C alpha is active and can be used in kinase assay. It is also a good biomarker for cancer diagnosis.

Formulation and Storage
Suppplied in 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA. This Product is stable at -70°C for at least 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

Synonym
cAMP-dependent protein kinase catalytic subunit alpha isoform 1; MGC102831; MGC48865 and PKACA.

Protein Sequence
MGNAAAAKKG SEQESVKEFL AKAKEDFLKK WESPAQNTAH LDQFERIKTL GTGSFGRVML
VKHKETGNHY AMKILDKQKV VKLKQIEHTL NEKRILQAVN FPFLVKLEFS FKDNSNLYMV
MEYVPGGEMF SHLRRIGRFS EPHARFYAAQ IVLTFEYLHS LDLIYRDLKP ENLLIDQQGY
IQVTDFGFAK RVKGRTWTLC GTPEYLAPEI ILSKGYNKAV DWWALGVLIY EMAAGYPPFF
ADQPIQIYEK IVSGKVRFPS HFSSDLKDLL RNLLQVDLTK RFGNLKNGVN DIKNHKWFAT
TDWIAIYQRK VEAPFIPKFK GPGDTSNFDD YEEEEIRVSI NEKCGKEFSE F

Background
PKA Cα, cAMP-dependent protein kinase catalytic subunit alpha, is involved in regulating cellular functions as metabolism, ion transport, and gene transcription. Free catalytic subunits of PKA can phosphorylate a wide variety of intracellular target proteins, such as NF-γB, NFAT5, CREB, GSK3α, etc. Mutation of the PKA Cα in mice leads to growth retardation and sperm dysfunction. Anti-PKA Cα autoantibodies are biomarkers for cancer diagnostic.

Image of SDS-PAGE /Western-blot