POU5F1, OCT3/4

• Species; Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 38.6 kDa.
• Gene Accession Number: BC117437.

Purification and Quality Control
The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified Pou5F1 is greater than 90% homogeneous based on SDS-PAGE analysis.

Applications
Marker for undifferentiation cells. May be used in stem cell related research. For Research use only.

Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw

Synonym
Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; Homo sapiens POU class 5 homeobox 1 (POU5F1) transcript variant 1.

Protein Sequence
MAGHLASDFA FSPPPGGGGD GPGGPEPGWV DPRTWLSFQG PPGGPGIGPG VGPGSEVWGI PPCPPPYEFC GGMAYCGPQV GVGLVPQGGL ETSQPEGEAG VGVESNSDGA SPEPCTVTPG AVKLEKEKLE QNPEESQDIK ALQKELEQFA KLLKQKRITL GYTQADVGLT LGVLFGKVFS QTTICRFEAL QLSFKNMCKL RPLLQKWVEE ADNNENLQEI CKAETLVQAR KRKRTSIENR VRGNLENLFL QCPKPTLQQI SHIAQQLGLE KDVVRVWFCN RRQKGKRSSS DYAQREDFEA AGSPFSGGPV SFPLAPGPHF GTPGYGSPHF TALYSSVPFP EGEAFPPVSV TTLGSPMHSN

Background
Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, class 5, transcription factor 1) is a protein that in humans is encoded by the POU5F1 gene. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. Oct-4 expression must be closely regulated; too much or too little will cause differentiation of the cells, and its expression is required to sustain cell self-renewal and pluripotency. Oct-3/4 is the most recognized marker for totipotent ES cells.

References
1, Niwa H, Miyazaki J, Smith AG (April 2000). Nat. Genet. 24 (4): 372–6.
2, Hochedlinger K, Yamada Y, et al. (2005). Cell 121 (3): 465–77.
3,Maherali, N.; et al. (2007). Cell Stem Cell 1 (1): 55–70.

---

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.