Purification and Quality Control The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Pou5F1 is greater than 90% homogeneous based on SDS-PAGE analysis.
Applications Marker for undifferentiation cells. May be used in stem cell related research. For Research use only.
Formulation and Storage The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw
Synonym Oct-3; Oct-4; OCT3; OCT4; OTF-3; OTF3; OTF4; Homo sapiens POU class 5 homeobox 1 (POU5F1) transcript variant 1.
Background Oct-4 (octamer-binding transcription factor 4) also known as POU5F1 (POU domain, class 5, transcription factor 1) is a protein that in humans is encoded by the POU5F1 gene. This protein is critically involved in the self-renewal of undifferentiated embryonic stem cells. Oct-4 expression must be closely regulated; too much or too little will cause differentiation of the cells, and its expression is required to sustain cell self-renewal and pluripotency. Oct-3/4 is the most recognized marker for totipotent ES cells.
References 1, Niwa H, Miyazaki J, Smith AG (April 2000). Nat. Genet. 24 (4): 372–6. 2, Hochedlinger K, Yamada Y, et al. (2005). Cell 121 (3): 465–77. 3,Maherali, N.; et al. (2007). Cell Stem Cell 1 (1): 55–70.
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
