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Lin28 (Cat# P2050)
Purification and Quality Control
The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Lin28 is greater than 95% homogeneous based on SDS-PAGE analysis.
Applications
May be used as RNAi assistance to restore the loss-of-function. For Research use only.
Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.
Synonym
LIN28, CSDD1, LIN28A, ZCCHC1, Protein lin-28 homolog A (Lin-28A) (Zinc finger CCHC domain-containing protein1.
Protein Sequence
MGSVSNQQFA GGCAKAAEEA PEEAPEDAAR AADEPQLLHG AGICKWFNVR MGFGFLSMTA RAGVALDPPV DVFVHQSKLH MEGFRSLKEG EAVEFTFKKS AKGLESIRVT GPGGVFCIGS ERRPKGKSMQ KRRSKGDRCY NCGGLDHHAK ECKLPPQPKK CHFCQSISHM VASCPLKAQQ GPSAQGKPTY FREEEEEIHS PTLLPEAQN
Background
Lin28 is a RNA-binding protein that belongs to a diverse family of structurally-related transcription factors. Lin28 is found abundantly in embryonic stem cells (ESCs), and to a lesser extent in placenta and testis. Lin28 has been shown to block let-7 microRNA processing and maturation, a necessary step in the differentiation of stem cells and certain cancer cell lines. Together with Sox2, Oct4, and Nanog, Lin28 can induce the reprogramming of primary human fibroblasts to a pluripotent state. Lin28 and other regulatory proteins can be introduced into cells by DNA transfection, viral infection, or microinjection. Protein transduction using TAT fusion proteins represents an alternative methodology for introducing proteins into primary as well as transformed cells. Recombinant human Lin28 is a 24 kDa protein containing 209 amino acid residues.
References
1, Richards M, Tan SP, et al. (2004).. Stem Cells 22 (1): 51–64.
2, Polesskaya A, Cuvellier S, et al. (May 2007). Genes Dev. 21 (9): 1125–38.
3, Lee YS, Kim HK, Chung S, et al. (2005).J. Biol. Chem. 280 (17): 16635–41.
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
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