RAR alpha-LBD (154-462)

• Species: Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 95%
• Molecular Weight: 36.4 kDa.
• Gene Accession Number: NM_000964.

Purification and Quality Control
The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified RAR-alpha-LBD is greater than 95% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)
1 unit equals 1 nanogram of purified protein. 20 unit is sufficient for a gel mobility shift assay; 100 units are sufficient for protein-protein interaction assays.

Applications
RARα can be applied in in vitro transcription assays, DNA-protein and protein-protein interaction assays.

Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym
RARA; NR1B1 and RAR.

Protein Sequence
SKESVRNDRN KKKKEVPKPE CSESYTLTPE VGELIEKVRK AHQETFPALC QLGKYTTNNS
SEQRVSLDID LWDKFSELST KCIIKTVEFA KQLPGFTTLT IADQITLLKA ACLDILILRI
CTRYTPEQDT MTFSDGLTLN RTQMHNAGFG PLTDLVFAFA NQLLPLEMDD AETGLLSAIC
LICGDRQDLE QPDRVDMLQE PLLEALKVYV RKRRPSRPHM FPKMLMKITD LRSISAKGAE
RVITLKMEIP GSMPPLIQEM LENSEGLDTL SGQPGGGGRD GGGLAPPPGS CSPSLSPSSN
RSSPATHSP

Background
Several members of nuclear receptor family are directly associated with human malignancies including breast cancer, prostate cancer and leukemia. The pathogenesis of each of these diseases is underpinned by the activities of a member of the superfamily; estrogen receptor-alpha (ER alpha) in breast cancer, androgen receptor (AR) in prostate cancer, and retinoic acid receptor alpha (RAR alpha) in acute promyelocytic leukemia (1). Retinoic acid receptors are important in the regulation of growth and differentiation of epithelial tissues, embryonic and central nervous system development and hematopoiesis (2). Retinoids mediate their effect by two classes of nuclear receptor proteins, the retinoic acid receptors (RARs) and the retinoid X receptors (RXRs), that each consists of three isotypes (α, β, and γ) encoded in separate genes (3). Upon dimerization with RXR, RARs can bind to specific enhancer sequences in the DNA, so-called retinoic acid response elements (RAREs), resulting in transcriptional activation of target genes in the presence of ligand (4). The retinoic acid receptor alpha gene is the target of chromosomal rearrangements in all cases of acute promyelocytic leukemia (APL). RAR alpha is a negative regulator of promyelocyte differentiation when not complexed with RA, and stimulates this differentiation when bound to RA (5).

Image of SDS-PAGE /Western-blot