α-RNA Pol II antibody is a mouse monoclonal antibody (ascites) without further purification.

The carboxy-terminal repeat domain (CTD) of the largest subunit of RNA pol II contains tandem repeats of a heptapeptide sequence Tyr-Ser-Pro-Thr-Ser-Pro-Ser which is highly conserved among eukaryotic organisms (1). There are two forms of RNA pol II in vivo, designated IIO, which is extensively phosphorylated at the CTD, and IIA, which is not phosphorylated. The IIA form preferentially enters the pre-initiation complex (PIC), whereas IIO is found in the elongating complex (2-3). The kinase activity of TFIIH can mediate CTD phosphorylation (4), although other kinases, including Cdc2 (5), Ctk1 (6), the Srb10-Srb11 kinase-cyclin pair (7), and P-TEFb (8), have also been implicated in CTD phosphorylation. A phosphatase responsible for the dephosphorylation of the CTD has also been identified (9). CTD phosphatase activity is regulated by TFIIB and TFIIF (10). The CTD has also been implicated in pre-mRNA processing, most likely functioning as a platform for the recruitment and assembly of factors involved in pre-mRNA processing (11).

Concentration: from 0.25 ml of ascites.

Specificity
α-RNA Pol II reacts with the CTD subunit in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:3000.

Storage
Aliquot and store at –20°C.

References:
1. Lu, H., et al., (1991) Proc. Natl. Acad. Sci. USA 88, 10004-10008
2. Dahmus, M.E. (1994) Prog. Nucleic Acid Res. Mol. Biol. 48, 143-179
3. O’Brian, T., et al., (1994) Nature 370, 75-77
4. Feaver, W. J., et al.,(1991) Cell 67, 1223-1230
5. Cisek, L.J., et al., (1989) Nature 339, 679-684
6. Lee, J. M., et al., (1991) Gene Expr. 1, 149-167
7. Liao, S. M., et al., (1995) Nature 374, 193-196
8. Marshall, N. F., et. al., (1996) J. Biol. Chem. 271, 27176-27183
9. Chambers, R.S., et al., (1994) J. Biol. Chem. 269, 26243-26248
10. Chambers, R. S., et al., (1995) J. Biol. Chem. 270, 14962-14969
11. McCracken, S., et al., (197) Nature 385, 357-361

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.