SC35, a member of the SR protein family, is an essential pre-mRNA splicing factor and can function as an essential splicing factor as well as an alternative splicing factor (1, 2). Phosphorylation of serine residues located within the SR domain directly regulates SC35 activity and compartmentalization of other SR splicing factors (3, 4). In addition to interacting with RNA and other splicing factors, SC35 has been shown to interact either directly or indirectly with the C-terminal domain (CTD) of the largest subunit of RNA polymerase II, thereby suggesting a potential role of SC35 in coordinating transcription and pre-mRNA splicing (5, 6).

Each vial contains 100 mg IgG in 0.1 ml of PBS.

Specificity
α-SC35 reacts with the SC35 protein in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:2000.

Storage
Aliquot and store at –20°C.

References:
1. Fu, X.D., et al., (1992) Science 256, 535-538
2. Zahler, A.M., et al., (1992) Genes & Dev. 6, 837-847
3. Gui, J.F., et al., (1994) Proc. Natl. Acad. Sci. USA 91, 10824-10828
4. Colwill, K., et al., (1996) EMBO J. 15, 265-275
5. Mortillaro, M.J., et al., (1997) Proc. Natl. Acad. Sci. USA 93, 8253-8257
6. Misteli, T., et al., (1997) Nature 387, 523-527

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.