Signal Transducer and Activator of Transcription 2

• Species; Human
• Expression Host: Baculovirus-insect cell
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 101.2 kDa.
• Gene Accession Number: NM_005419.

Purification and Quality Control

The Flag-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified STAT2 is greater than 90% homogeneous based on SDS-PAGE analysis. Purified protein contains no detectable proteases, DNase and RNase activity.

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays.

Applications

Recombinant STAT2 (1-851) can be used for 1) in vitro transcription; 2) gel mobility shift assay and 3) for protein-protein interaction assay.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Protein Sequence

MAQWEMLQNL DSPFQDQLHQ LYSHSLLPVD IRQYLAVWIE DQNWQEAALG SDDSKATMLF FHFLDQLNYE CGRCSQDPES LLLQHNLRKF CRDIQPFSQD PTQLAEMIFN LLLEEKRILI QAQRAQLEQG EPVLETPVES QQHEIESRIL DLRAMMEKLV KSISQLKDQQ DVFCFRYKIQ AKGKTPSLDP HQTKEQKILQ ETLNELDKRR KEVLDASKAL LGRLTTLIEL LLPKLEEWKA QQQKACIRAP IDHGLEQLET WFTAGAKLLF HLRQLLKELK GLSCLVSYQD DPLTKGVDLR NAQVTELLQR LLHRAFVVET QPCMPQTPHR PLILKTGSKF TVRTRLLVRL QEGNESLTVE VSIDRNPPQL QGFRKFNILT SNQKTLTPEK GQSQGLIWDF GYLTLVEQRS GGSGKGSNKG PLGVTEELHI ISFTVKYTYQ GLKQELKTDT LPVVIISNMN QLSIAWASVL WFNLLSPNLQ NQQFFSNPPK APWSLLGPAL SWQFSSYVGR GLNSDQLSML RNKLFGQNCR TEDPLLSWAD FTKRESPPGK LPFWTWLDKI LELVHDHLKD LWNDGRIMGF VSRSQERRLL KKTMSGTFLL RFSESSEGGI TCSWVEHQDD DKVLIYSVQP YTKEVLQSLP LTEIIRHYQL LTEENIPENP LRFLYPRIPR DEAFGCYYQE KVNLQERRKY LKHRLIVVSN RQVDELQQPL ELKPEPELES LELELGLVPE PELSLDLEPL LKAGLDLGPE LESVLESTLE PVIEPTLCMV SQTVPEPDQG PVSQPVPEPD LPCDLRHLNT EPMEIFRNCV KIEEIMPNGD PLLAGQNTVD EVYVSRPSHF YTDGPLMPSD F

Background

The signal transducer and activator of transcription (STAT) proteins play a crucial role in the regulation of gene expression by mediating transcriptional responses to many cytokines and growth factors (1). The antiviral responses of IFN-α/β rely on the transcriptional activities of STAT1 (cat# R1072) and STAT2 proteins (2,3). The chromatin immunoprecipitation and DNA microarray analysis (Chip-chip) demonstrated that STAT2 (and STAT1) bound to chromosome 22 after IFN stimulation (4, 5). STAT2 activity can be regulated by tyrosine phosphorylation that is catalyzed by a receptor associated JAK kinase (6).

Image of SDS-PAGE /Western-blot