Transcription Factor IIA, p55 subunit

• Species: Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 43.6 kDa.
• Gene Accession Number: NM_015859.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified p55 subunit of TFIIA is greater than 95% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 20 units are sufficient for in vitro transcription assay and 100 units are sufficient for protein-protein interaction assays.

Applications

Recombinant TFIIA can be used to super-shift TBP-DNA complex in a gel mobility shift assay and to stimulate activator-dependent transcription in vitro(3, 5). For Research use only.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym

MGC129969; MGC129970; TF2A1; TFIIA; Homo sapiens general transcription factor IIA, 1, 19/37kDa(GTF2A1).

Protein Sequence

MANSANTNTV PKLYRSVIED VINDVRDIFL DDGVDEQVLM ELKTLWENKL MQSRAVDGFH SEEQQLLLQV QQQHQPQQQQ HHHHHHHQQA QPQQTVPQQA QTQQVLIPAS QQATAPQVIV PDSKLIQHMN ASNMSAAATA ATLALPAGVT PVQQILTNSG QLLQVVRAAN GAQYIFQPQQ SVVLQQQVIP QMQPGGVQAP VIQQVLAPLP GGISPQTGVI IQPQQILFTG NKTQVIPTTV AAPTPAQAQI TATGQQQPQA QPAQTQAPLV LQVDGTGDTS SEEDEDEEED YDDDEEEDKE KDGAEDGQVE EEPLNSEDDV SDEEGQELFD TENVVVCQYD KIHRSKNKWK FHLKDGIMNL NGRDYIFSKA IGDAEW

Background

The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α subunit), 19 kDa (β subunit) and 12 kDa (γ subunit). The α and β subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ subunit), retains native TFIIA activity(1-4). For reconstituted TFIIA, containing all three subunits please see P1032 in our catalog.