The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified Transcription Factor IIA, p12 subunit is greater than 95% homogeneous based on SDS-PAGE analysis.
Unit Definition (Activity)
1 unit equals 1 nanogram of purified protein. 1 unit is sufficient for a gel mobility shift assay in a 20 µl reaction; 100 units are sufficient for protein-protein interaction assays.
Applications
Recombinant p12 in combination with other subunits of TFIIA has been applied for in vitro transcription assays, gel mobility shift assays and protein-protein interaction assays (1, 4).
Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.
p12 is a small subunit (γ) of the transcription factor IIA and has been shown to be required for both basal and activated transcription. Recombinant p12, along with two other subunits (α and β) can potentiate transcriptional activation, whereas p12 along with the β subunit is able to function in anti-repression (1-3). For reconstituted TFIIA, containing all three subunits, please see P1032 in our catalog.
References
1. DeJong, J. et al., (1995) Proc. Natl. Acad. Sci. USA 92, 3313-3317 2. Ozer, J. et al., (1994) Genes & Dev. 8, 2324-2335 3. Sun, X. et al., (1994) Genes & Dev. 8, 2336-2348
4. Ma, D. et al., (1996) Proc. Natl. Acad. Sci. USA 93, 6583-6588
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
