1 unit equals 1 nanogram of purified protein. 20 units are sufficient for an in vitro reconstituted transcription assay in the presence of 34-kDa subunit; 100 units are sufficient for a protein-protein interaction assay. variable in different lots. For research use only.
Formulation and Storage
This protein was kept in 20 mM Tris-Cl (pH 8.0), 20% Glycerol, 100 mM KCl, 1 mM DTT and 0.2 mM EDTA. Always keep in -20°c, avoid freeze thaw cycles.
The human Transcription Factor IIE (TFIIE) is composed of 56 kDa (α) and 34 kDa (β) subunits and is shown to be a heterotetramer (1, 2). The 56-kDa subunit contains a region similar to a zinc-binding domain and a region sharing homology with the catalytic loop of a kinase domain (3). TFIIE binds to RNA polymerase II in solution and joins the preinitiation complex probably concomitant with RNA polymerase II and TFIIF (4). The His tagged recombinant protein is purified from an E. coli strain that contains the coding sequence of human TFIIE α subunit under the control of T7 promoter (5). Although the 56-kDa subunit of the TFIIE contains a zinc-binding domain like region, both subunits are required to reconstitute the functional transcription factor.
References
1. Ohkuma, Y. et al., (1990) Proc. Natl. Acad. Sci. USA 87, 9163-9167 2. Inostroza, J. et al., (1991) J. Biol. Chem. 266, 9304-9308 3. Peterson, M. et al., (1991) Nature 354, 369-373. 4. Flores, O. et al., (1990) J. Biol. Chem. 265, 5629-5634
5. Maldonado, E. et al., (1996) Methods Enzymol. 274, 72-100
DISCLAIMER
This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.
