α-TFIIA antibody is an affinity-purified rabbit polyclonal antibody raised against the recombinant full-length p55 subunit of the TFIIA complex.

The transcription factor IIA (TFIIA) has been shown to bind to the TBP-DNA complex and to increase the affinity of TBP for the TATA element. Human TFIIA consists of three subunits of 35 kDa (α subunit), 19 kDa (β subunit) and 12 kDa (γ subunit). The α and β subunits are derived from the product, p55, of a single gene by an unknown mechanism. However, recombinant p55, in combination with a 12 kDa subunit (γ subunit), retains native TFIIA activity (1-4).

Each vial contains 100 μg IgG in 0.1 ml of PBS.

Specificity
α-TFIIA reacts with the p55 protein in HeLa nuclear extract by Western Blotting. Recommended dilution range for Western blot analysis: 1:2000-1:5000. Recommended starting dilution: 1:2000.

Storage
Aliquot and store at –20°C.

References:
1. Buratowski, S. et al., (1989) Cell 56, 549-561
2. Ranish, J. et al., (1991) J. Biol. Chem. 266, 19320-19327
3. DeJong, J. et al., (1993) Genes & Dev. 7, 2220-2234
4. Ma, D. et al., (1993) Genes & Dev. 7, 2246-2257

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.