TNF-alpha (Low Endotoxin)

• Species: Human
• Expression Host: E.coli
• Tag: His-tag
• Purity: 95%
• Molecular Weight: 21 kDa.
• Gene Accession Number: NM_000594.

Purification and Quality Control
The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns.
The purified TNF-alpha is greater than 95% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)
1 unit equals 1 nanogram of purified protein.

Applications
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Formulation and Storage
The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Synonym
DIF; TNF-alpha; TNFA and TNFSF2.

Protein Sequence
VRSSSRTPSD KPVAHVVANP QAEGQLQWLN RRANALLANG VELRDNQLVV PSEGLYLIYS
QVLFKGQGCP STHVLLTHTI SRIAVSYQTK VNLLSAIKSP CQRETPEGAE AKPWYEPIYL
GGVFQLEKGD RLSAEINRPD YLDFAESGQV YFGIIAL

Background
Tumor necrosis factor alpha (TNFα or TNFSF2) is produced by many different cell types including activated monocytes, fibroblasts, endothelial cells, macrophages, T-cells, B-lymphocytes, granulocytes, smooth muscle cells, eosinophilis, chondrocytes and other cell types (1). It functions as a cytokine involved in cell proliferation, differentiation, apoptosis, lipid metabolism and coagulation (2, 3). High level of TNFα leads to the development of inflammatory responses that are hallmarks of many diseases including autoimmune diseases, insulin resistance and cancer (4, 5). The active form of TNFa (18kDa) is generated by the cleavage of an Ala-Val bond between residues 76-77 of the precursor protein (6).

Image of SDS-PAGE /Western-blot