Upstream Stimulatory Factor 1

• Species: Human
• Expression Host: E. coli
• Tag: His-tag
• Purity: 90%
• Molecular Weight: 35.1 kDa.
• Gene Accession Number: NM_007122.

Purification and Quality Control

The His-tag recombinant protein is purified by affinity chromatography in combination with FPLC columns. The purified USF1 is greater than 90% homogeneous based on SDS-PAGE analysis.

Unit Definition (Activity)

1 unit equals 1 nanogram of purified protein. 5-50 units are sufficient for DNA binding assays. 50-500 units are required for an in vitro transcription assay and 100 units are required for protein-protein interaction assay.

Applications

Recombinant USF1 can be used for: 1) in vitro transcription assays; 2) DNA binding assays (gel mobility shift assys and DNA footprint assays), and 3) protein-protein interaction assays.

Formulation and Storage

The protein is in 20mM Tris-HCl pH7.9,100mM NaCl, 0.2mM EDTA, 1mM DTT and 20% glycerol. Stored at -70°C before use. Avoid repeated freeze thaw cycles.

Protein Sequence

MKGQQKTAET EEGTVQIQEG AVATGEDPTS VAIASIQSAA TFPDPNVKYV FRTENGGQVM YRVIQVSEGQ LDGQTEGTGA ISGYPATQSM TQAVIQGAFT SDDAVDTEGT AAETHYTYFP STAVGDGAGG TTSGSTAAVV TTQGSEALLG QATPPGTGQF FVMMSPQEVL QGGSQRSIAP RTHPYSPKSE APRTTRDEKR RAQHNEVERR RRDKINNWIV QLSKIIPDCS MESTKSGQSK GGILSKACDY IQELRQSNHR LSEELQGLDQ LQLDNDVLRQ QVEDLKNKNL LLRAQLRHHG LEVVIKNDSN

Background

Human Upstream Stimulatory Factor 1 (USF1) regulates the transcription of many genes involved in lipid and glucose homeostasis and co-localizes with familial combined hyperlipidemia (FCHL) and type 2 diabetes on chromosome 1q22-23. (1-3). The USF1 protein can activate transcription through pyrimidine-rich initiator (Inr) elements and E-box motifs. It is a basic helix-loop-helix leucine zipper (b-HLH-ZIP) protein that shares a common DNA-binding specificity with the c-Myc oncoproteins (4-6).

References

1.Putt, W. et al., (2004) Hum. Mol. Genet. 13, 1587-1597.
2.Pajukanta, P. et al., (2004) Nat. Genetics 36, 371-376.
3.Du, H. et al., (1993) EMBO J. 12, 501-511.
4.Gregor, PD. Et al., (1990) Genes & Dev. 4, 1730-1740.
5.Ge, Y. et al., (2003) J. Biol. Chem. 278, 49901-49910.

6.Sawadogo, M. et al., (1985) Cell 43, 165-175.

DISCLAIMER

This products is recommended For RESEARCH USE ONLY and is Not qualified for Use in Diagnostic or Therapeutic Procedures.